![]() It may cause chromatin to be squeezed out of nuclei. Distortion can be caused by the slightest compression of tissue and can provide difficulties in diagnosis. Ī crush artifact is an artificial elongation and distortion seen in histopathology and cytopathology studies, presumably because of iatrogenic compression of tissues. Different techniques including freeze-fracturing and cell fractionation may be used to overcome the problems of artifacts. Staining can cause the appearance of solid chemical deposits that may be seen as structures inside the cell. In electron microscopy, distortions may be produced in the drying out of the specimen. In light microscopy, artifacts may be produced by air bubbles trapped under the slide's cover slip. ![]() In microscopy, an artifact is an apparent structural detail that is caused by the processing of the specimen and is thus not a legitimate feature of the specimen. Photo: Heiti Paves from Tallinn University of Technology. However, an intact thale cress stamen does not have such collar, this is a fixation artifact: the stamen has been cut below the picture frame, and epidermis (upper layer of cells) of stamen stalk has peeled off, forming a non-characteristic structure. The picture shows among other things a nice red flowing collar-like structure just below the anther. The differing cases of visual artifacting can also differ between scheduled task(s).Ĭonfocal laser scanning fluorescence micrograph of thale cress anther (part of stamen).
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